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Database of oligomerization domains from lambda experiments |
Doodle is a database that was developed to store and distribute information about the protein oligomerization domains that are encoded by various genomes. The protein oligomerization domains described here were found using the lambda repressor fusion system. Doodle uses a schema that is based on EnsEMBL, while also utilizing bioperl modules to both store and retrieve data. The frontend was developed entirely in perl, while the backend utilizes MySQL. GMOD was used to develop the genomic view. This project is being funded by the NIH (grant GM063652-01).
Repressor fusions have been used since their original description in the early 1990's to characterize and map protein-protein interactions from various organisms. Repressor fusion literature from the Hu lab in PubMed.
| Doodle Team | Doodle Alumni |
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Gwen Knapp - Graduate Student Gregory Munoz - Graduate Student Adrienne Zweifel - Graduate Student Reagan Viney - Undergraduate Student Melissa Fowler - Undergraduate Student Theo Judd - Undergraduate Student Jon Minor - Research Assistant James C. Hu - Principal Investigator |
Leonardo Mariño-Ramírez - Graduate Student Xuemei Tang - Graduate Student Lisa Dandridge - Postdoctoral Research Associate Brian Hatten - Undergraduate Student Emily Joyner - Research Assistant Nicola Reading - Undergraduate Student |
This section contains documentation for the Hulab.
This section contains repressor fusion protocols.
1. Mariño-Ramírez, L., Minor, J.L., Reading, N. and Hu J.C. (2004) Identification and mapping of self-assembling protein domains encoded by the Escherichia coli K-12 genome by use of lambda repressor fusions. J. Bacteriol. 186:1311-1319. [PubMed] [PDF]
2. Mariño-Ramírez, L. and Hu J.C. (2002) Isolation and mapping of self-assembling protein domains encoded by the Saccharomyces cerevisiae genome using lambda repressor fusions. Yeast 19:641-650. [PubMed] [PDF]
3. Mariño-Ramírez, L. and Hu, J.C. (2001) Using lambda repressor fusions to isolate and characterize self-assembling domains, in Protein-Protein Interactions: A Laboratory Manual, (Golemis, E., and Serebriiskii, I. ed.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. [CSHL Press]
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The Doodle website is maintained by the Hu Lab in the Dept. of Biochemistry and Biophysics at Texas A & M University. For problems or comments on this website contact Jim Hu |
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